ABSTRACT
Objective To investigate the effects of bradykinin (BK) on the proliferation of rabbit corneal endothehal ceils (RCECs) and the expression of tight junction-related proteins zonula occludens-1 (ZO-1) and zonula occludens-1-associated nucleic-acid-binding protein (ZONAB),and to explore the underlying mechanisms of BK on cell proliferation in corneal endothelium.Methods RCECs at logarithmic growth phase were treated with different concentrations of BK (0.01,0.1,1,10 μmol · L-1) BK group,with the controls left untreated.Morphological changes of cells in each group were examined under phase-contrast microscope,and MTT assays were used to detect cell proliferation at 24 h,48 h,72 h,96 h after BK treatment.And,at 72 h,the expression levels of ZO-1 and ZONAB protein were determined by Western blot.Results After 72 h of treatment,the cells in each group were fused into pieces and closely linked into a monolayer;but after 96 h,the growth of the cells was restricted,with the intercellular space become larger and the cells exfoliated.Compared with the control group,BK induced a significant increase of absorbance value and cell viability,and the differences were statistically significant (all P ＜ 0.001),and the promoting effects showed a concentration-dependent manner,and 1 μmol · L-1 BK demonstrated the strongest regulative effect (P ＜ 0.001).Western blot results showed that BK upregulated the expression of ZO-1 and ZONAB protein in a concentration-dependent manner.Conclusion BK can stimulate the proliferation of RCECs in a time-and concentration-dependent manner,and the mechanisms are probably associated with ZO-1/ZONAB-mediated signaling pathway.
ABSTRACT
Optical coherence tomography (OCT),as a noncontact,innovative technology,can produce high-resolution images of ocular tissues.This technology is originally used for posterior segment in ophthalmology,which were widely utilized for the anterior segment at first.The development of anterior segment OCT (AS-OCT) technology enables the precise visualization of anterior segment structure.Moreover,ultrahigh-resolution OCT (UHR-OCT) has been helpful for diagnosis and management of corneal and anterior segment diseases,including quantification of tear film for dry eye,authentication of ocular surface squamous neoplasia (OSSN),pterygium,keratoconus,traumatic corneal injuries,etc.Besides,UHR-OCT can be also used for planning and performing surgery for anterior segment,such as corneal transplantation,and monitoring of epithelial wound healing and postoperative course as well.Moreover,this technology can help understand the physiology and pathophysiology of the anterior segment structure.Therefore,this review will give a review on the application of UHR-OCT for diagnosis and management of corneal and anterior segment disorders.